Determination of Proteins Using Biuret and Lowry Assay Techniques Essay

Assurance of Proteins Using Biuret and Lowry Assay Techniques - Essay Example Protein examine is basic in the examination of rural, modern and biotechnological items. As contended by Bama et al. (2010), it is additionally significant for research particularly in examination of catalysts, lectins and antibodies. This paper covers two sorts of measures utilized in quantitating absolute proteins. These incorporates the biuret and lowry strategies. Biuret test, which is the least delicate measure is among the coulometric strategies (Quereshi et al. 2010). It is generally utilized because of its straightforwardness and less powerlessness to compound obstruction. The measure is reliant on polypeptide chelation of cupric iron in solid antacid. As per Mizuta et al. (2005), most biuret measures are utilized in tests containing 1 to 10mg protein/ml, which is then weakened five-overlap by different reagents to shape profound purple shading. Then again, the Lowry technique is a colorimetric measure that depends on folin-ciocalteau reagent and cupric particles of phenolic gatherings (Muyonga, Cole and Duodu, 2004). It is a well known protein estimation system despite the fact that exceptionally powerless to perceiving exacerbates that meddle and misshape dissolvability of insoluble proteins. The measure begins with copper particle complex that has peptide securities, which are balanced out by tartrate in soluble condition famous known as biuret chromophore. Gornall, Bardawill and David (1949) called attention to that biuret response is decreased under basic states of folin-ciocalteu reagent. Copper particles are utilized to upgrade the decrease procedure. Notwithstanding, the standard chromogenic bunches comprise of the peptide linkages that diminished blue molybdotungstates, which catalyzes polar amino acids, tyrosine and tryptophan. In any case, the affectability of this test depends on protein sythesis and results of synthetic concoctions response coming about to the heteropolymolybdenum blue arrangement in the wake of being in absorbance state of around 750nm, a frequency that is out of scope of many meddling hues (Layne, 1957). In these two examinations, the fundamental law of light ingestion, prominently knows as Beer-Lambert law is utilized to clarify the straight connection between protein (collagen) focus and absorbance (Cliche, Amiot and Avezard, 2003). The yield of collagen is determined utilizing the accompanying lines condition: Y=(VxC)/W Where; Yis the yield of collagen in mg/g Vis the volume of collagen arrangement in ml C is the grouping of the inferred arrangement in mg/ml Wis the lyophilized load in g Materials utilized: 1. Protein test of obscure focus 2. Standard BSA 3. Refined water 4. Lowry reagent 5. Test tubes 6. Name 7. Test tube rack 8. Pipettes 9. Pipette bulb 10. Vortex blender 11. Spectrophotometer 12. Cuvettes 13. Gelatin : 100â µg cm-3 14. Globulin: 100â µg cm-3 15. egg whites: 200â µg cm-3 Methods Lowry Technique: Procedure: 1. Get ready examples with up to 100 ?g of protein 2. Mark the 9 test tubes as (1 to 10) and spot them in a test tube rack. 3. Include water as gave in the directions. 4. Plan weakened Folin-Ciocalteu reagent and the Assay Mix. 5. Include 0.5cm3 of the protein answer for tubes (2 to 10). 6. Add gelatin answer for tube 7 and 8 as it were. 7. At that point add 2.5cm3 of arrangement D to each cylinder and blend well and leave the blend at room temperature for around 10 minutes.

Answer the three questions by writing at least a page stating your Essay

Answer the three inquiries by composing in any event a page expressing your conclusion on each question - Essay Example I, similar to greater part of Americans bolster capital punishment. I have various explanations behind supporting capital punishment. The principal explanation behind supporting it is educated by the conviction that retaliation is a significant solution for wrongdoing submitted. Specifically, I consider capital punishment as a characteristic human reaction to horrifying violations, for example, murder. In the event that the respondent is seen as liable of removing an existence of another person, at that point the life of that specific litigant ought to likewise be taken, as that is the main discipline that measures to the wrongdoing. The second purpose behind supporting capital punishment is educated by the conviction that it will go about as an impediment for different people who might be thinking about or may consider later on to perpetrate comparative wrongdoing. The seriousness of capital punishment will make it more outlandish for an individual to carry out a wrongdoing that cal ls for such a discipline. The last purpose behind supporting capital punishment is debilitation; this discipline guarantees that an indicted wrongdoer won't get an opportunity of carrying out a similar wrongdoing again as society will dispose of the person in question totally. #2: Do indicted killers invest an excess of energy in death row before their sentences are completed? The length that indicted killers spend waiting for capital punishment before the completing of their sentences has risen as one of the subject of enthusiasm with respect to capital punishment, as of late. It is upsetting to know from the accessible information that some death row prisoners have gone through more than 20 years waiting for capital punishment anticipating execution. What is additionally upsetting about this circumstance is the way that during this deferred time, these detainees are commonly disengaged from different detainees, just as being rejected from jail business and instructive projects. Wh ile there are the individuals who imagine that indicted killers don't invest an excessive amount of energy in death row before their sentences are completed, I accept that they do. I believe that killers do invest an excessive amount of energy in death row, a reasoning that is educated by accessible information and insights. As indicated by Death Penalty Information Center, the length that the killers in the United States spend waiting for capital punishment has gotten progressively longer as of late. Utilizing information from the Bureau of Justice Statistics, Death Penalty Information Center expresses that the normal time among condemning and execution of killers in the United States is 178 months (Death Penalty Information Center para 7). This information affirms that surely killers invest a lot of energy in death row before their sentences are completed. This long length takes colossal cost for the detainees, their families, and on the citizens. While capital punishment advocate s and adversaries the same concur that cautious survey that may require longer time is basic, they concur that an excess of time is destructive and equity framework should discover methods of decreasing this unsatisfactory long span. #3: Can you be a decent American and not vote? Customarily, casting a ballot rights have been considered as key to fair citizenship. Citizenship by and large suggests a heap of social, political, and social liberties, all of which can significantly be reflected through democratic. Basically, citizenship is a full enrollment status in a self-overseeing country. This origination includes the constituent rights where residents take part in aggregate self-government through deciding in favor of agents or legitimately. Regularly, it is through democratic fo

Basic Parts of the Brain and Their Responsibilities

Essential Parts of the Brain and Their Responsibilities The scarecrow required it, Einstein had an amazing one, and it can store a mess of data. What is it you state? Why, the cerebrum of course. The mind is the control focal point of the body. Think about a phone administrator who answers approaching calls and guides them to where they have to go. Correspondingly, your mind goes about as an administrator by sending messages to and accepting messages from everywhere throughout the body. The mind forms the data it gets and guarantees thatâ messages are coordinated to their appropriate goals. Neurons The cerebrum is made out of particular cells called neurons. These cellsâ are the fundamental unit of the sensory system. Neurons send and get messages through electrical driving forces and concoction messages. Concoction messages are known as synapses and they can either repress cell movement or cause cells to become excitable.â Cerebrum Divisions The cerebrum is one of the biggest and most significant organs of the human body. Tipping the scales at around three pounds, this organ is secured by a three-layered defensive film called the meninges. The brainâ has a wide scope of duties. From organizing our development to dealing with our feelings, this organ does it all. The cerebrum is made out of three primary divisions: the forebrain, brainstem, and hindbrain. Forebrain The forebrain is the most mind boggling of the three sections. It enables us to feel, learn, and recall. It comprises of two sections: the telencephalon (contains the cerebral cortex and corpus callosum) and the diencephalon (contains the thalamus and nerve center). The cerebral cortex permits us to comprehend the hills of data we get from surrounding us. The left and right areas of the cerebral cortex are isolated by a thick band of tissue called the corpus callosum. The thalamus goes about as a phone line of sorts, permitting data to break through to the cerebral cortex. It is likewise a segment of the limbic system,â whichâ connects zones of the cerebral cortex that are associated with tactile observation and development with different pieces of the cerebrum andâ spinal cord. The nerve center is significant for managing hormones, craving, thirst, and excitement. Brainstem The brainstem comprises of the midbrain and the hindbrain. Similarly as the name proposes, the brainstem looks like the stem of a branch. The midbrain is the upper piece of the branch that is associated with the forebrain. This locale of the cerebrum sends and gets data. Information from our faculties, for example, the eyes and ears, are sent to this region and afterward coordinated to the forebrain. Hindbrain The hindbrain makes up the lower part of the brainstem and comprises of three units. The medulla oblongata controls automatic capacities, for example, processing and relaxing. The second unit of the hindbrain, the pons, additionally helps with controlling these capacities. The third unit, the cerebellum, is answerable for the coordination of development. Those of you who are honored with extraordinary dexterity have your cerebellum to thank. Mind Disorders As you can envision, we all craving a mind that is solid and capacities appropriately. Shockingly, there are some who experience the ill effects of neurological issue of the mind. A couple of these disarranges include: Alzheimers sickness, epilepsy, rest disorders,â and Parkinsons malady.

Persuasive Speech for the Adoption of Organic Food into the Lunch Essay

Powerful Speech for the Adoption of Organic Food into the Lunch Program of the School - Essay Example Serving natural and normal food during our lunch would already be able to have an enormous effect in the advancement of our wellbeing and condition. My name is _________(your name over the line) and I am only a concerned understudy with a smart thought about our wellbeing and condition who additionally really thinks about my kindred understudies and my school. II. Body We may not know it, yet on the off chance that we are not specific about the sort and nature of the food that is served to us in the school cafeteria, they may really be serving us Frankenstein nourishments or hereditarily modified food sources. This isn't to be faulted the bottle overseers in light of the fact that a significant number of the nourishments sold at our goods are in truth hereditarily altered and it is hard to make sense of whether they are natural or not (Kenner, 2008). (Tell’em part) Genetically altered nourishments are as of now common. They are a result of the factorization of our food industr y that looks to deliver food in scale, but of lower quality than those that are normally developed by our ranchers. Hereditarily changed food sources are likewise peppered with synthetics so don't be shocked in the event that you despite everything don't feel supported after an abundant dinner in light of the fact that the greater part of the food you ate was not so much food yet were for the most part made out of synthetic concoctions (hurt). (damage, essentialness and cause) This isn't to state that common nourishments are now gone and completely supplanted by hereditarily changed nourishments. ... Kenner didn't mince with words in the presentation of his narrative film that GMO’s are truth be told, just presents an idea of a food in light of the fact that without anyone else, GMOs are not so much a food considering the fake sources of info that was consolidated during the procedure of its creation. Note that I utilized the term â€Å"production† and not â€Å"growing† simply like what is done in the homesteads in light of the fact that the industrialization of food can be compared more to a production line than to a ranch that â€Å"produces† food instead of â€Å"grow† them. GMO is ever present in the present servings of our cafeteria through its cheap food servings. I comprehend that my kindred understudies love quick nourishments on account of its taste and comfort. I don't care to repudiate just that quick nourishments are not beneficial both to our body and condition. Specialists concur, as announced by Rosencheck that the utilization of quick nourishments which are served large which have high glycemic loads add to the increasing pace of corpulence in the USA (2008). This was bolstered by the investigation of Currie whose review discoveries recommend that the expansion in the gracefully of drive-through joints significantly affect weight (2010). The negative wellbeing results of the increasing weight rates as of now are apparent. Paces of diabetes (the majority of which is type 2, which is to a great extent because of weight, less than stellar eating routine, and physical idleness) rose half somewhere in the range of 1990 and 2000 (Mokdad et al, 2001). Condition insightful, a similar quick nourishments that we like to eat are not additionally solid. We may not know it yet we additionally eat oil when we devour mechanical food. In general, the creation of the crude materials of the cheap food industry chugs up oil fuel of around 40,000 gallons for each year. This

Cloning :: essays research papers

Cloningâ â â â â What is a Clone? A clone is a gathering of hereditarily indistinguishable cells. For instance, tumors are clones of cells inside a living being on the grounds that they comprise of numerous copies of one changed cell. Another sort of clone happens inside a cell. Such a clone is comprised of gatherings of indistinguishable structures that contain hereditary material, for example, mitochondria and chloroplasts. A portion of these structures, called plasmids, are found in certain microscopic organisms and yeasts. Procedures of hereditary building empower researchers to join a creature or plant quality with a bacterial or yeast plasmid. By cloning such a plasmid, geneticists can create numerous indistinguishable duplicates of the quality. Employments of Cloning: Â Â Â Â Â Researchers said the cloning of creatures, particularly those that have been hereditarily altered in specific manners, could have various clinical, horticultural, and modern applications. For instance, cloning could bring about the large scale manufacturing of hereditarily adjusted cows that emit important medications into their milk. Be that as it may, the cloning of creatures showed that it may likewise be conceivable to clone people. A significant part of the open communicated repugnance toward the possibility of human cloning, and a few government officials promised to ban it. Its defenders, be that as it may, considered human to be as an approach to help individuals, for example, by permitting barren couples to have kids. Early Scientific Experiments of Cloning: Researchers have for quite some time been captivated by the chance of misleadingly cloning creatures. Truth be told, individuals have known since antiquated occasions that simply cutting them into two pieces can clone a few spineless creatures, for example, worms and starfish. Each piece develops into a total life form. The cloning of vertebrates (creatures with spines) is considerably more hard to clone. The initial phase in the cloning the mind boggling life forms (vertebrates) came in the 1950's with tests done on frogs. Â Â Â Â Â In 1952, Robert Briggs and Thomas King, researcher at the Institute for Cancer Research (presently the Fox Chase Cancer Center) in Philadelphia, built up a cloning technique called atomic transplantation, or atomic exchange, which was first proposed in 1938 by the German researcher Hans Spemann. In this strategy, the core - the cell structure that contains the vast majority of the hereditary material and that controls development and improvement - is expelled from an egg cell of a living being, a system known as enucleation. The core from a body cell of another living being of similar species is then put into the enucleated egg cell. Sustained by the supplements in the rest of the piece of the egg cell, an undeveloped organism (a living being preceding birth) starts developing. Cloning :: expositions explore papers Cloningâ â â â â What is a Clone? A clone is a gathering of hereditarily indistinguishable cells. For instance, tumors are clones of cells inside a creature since they comprise of numerous copies of one changed cell. Another sort of clone happens inside a cell. Such a clone is comprised of gatherings of indistinguishable structures that contain hereditary material, for example, mitochondria and chloroplasts. A portion of these structures, called plasmids, are found in certain microbes and yeasts. Methods of hereditary building empower researchers to consolidate a creature or plant quality with a bacterial or yeast plasmid. By cloning such a plasmid, geneticists can deliver numerous indistinguishable duplicates of the quality. Employments of Cloning: Â Â Â Â Â Researchers said the cloning of creatures, particularly those that have been hereditarily adjusted in specific manners, could have various clinical, agrarian, and mechanical applications. For instance, cloning could bring about the large scale manufacturing of hereditarily changed cows that discharge important medications into their milk. Yet, the cloning of creatures demonstrated that it may likewise be conceivable to clone people. A significant part of the open communicated aversion toward the possibility of human cloning, and a few lawmakers promised to ban it. Its defenders, notwithstanding, considered human to be as an approach to help individuals, for example, by permitting barren couples to have youngsters. Early Scientific Experiments of Cloning: Researchers have for some time been charmed by the chance of falsely cloning creatures. Truth be told, individuals have known since antiquated occasions that simply cutting them into two pieces can clone a few spineless creatures, for example, night crawlers and starfish. Each piece develops into a total living being. The cloning of vertebrates (creatures with spines) is substantially more hard to clone. The initial phase in the cloning the perplexing creatures (vertebrates) came in the 1950's with tests done on frogs. Â Â Â Â Â In 1952, Robert Briggs and Thomas King, scholars at the Institute for Cancer Research (presently the Fox Chase Cancer Center) in Philadelphia, built up a cloning technique called atomic transplantation, or atomic exchange, which was first proposed in 1938 by the German researcher Hans Spemann. In this strategy, the core - the cell structure that contains the majority of the hereditary material and that controls development and advancement - is expelled from an egg cell of a creature, a technique known as enucleation. The core from a body cell of another creature of similar species is then set into the enucleated egg cell. Sustained by the supplements in the rest of the piece of the egg cell, an incipient organism (a living being preceding birth) starts developing.

Can You Change Your Personality

Can You Change Your Personality Theories Personality Psychology Print Can You Change Your Personality? By Kendra Cherry facebook twitter Kendra Cherry, MS, is an author, educational consultant, and speaker focused on helping students learn about psychology. Learn about our editorial policy Kendra Cherry Updated on June 24, 2019 Benjamin A. Peterson / Getty Images More in Theories Personality Psychology Myers-Briggs Type Indicator Behavioral Psychology Cognitive Psychology Developmental Psychology Social Psychology Biological Psychology Psychosocial Psychology Is it really possible to change your personality or are our basic personality patterns fixed throughout life? While self-help books and websites often tout plans you can follow to change your habits and behaviors, there is a persistent belief that our underlying personalities are impervious to change. The Austrian psychoanalyst Sigmund Freud suggested that personality was largely set in stone by the tender age of five. Even many modern psychologists suggest that overall personality is relatively fixed and stable throughout life. But what if you want to change your personality? Can the right approach and hard work lead to real personality change, or are we stuck with undesirable traits that hold us back from achieving our goals? Is personality permanent? The desire to alter personality is not uncommon. Shy people might wish they were more outgoing and talkative. Hot-tempered individuals might wish they could keep their cool in emotionally charged situations. At many points in your life, you may find that there are certain aspects of your personality that you wish you could change. You might even set goals and work toward tackling those potentially problematic traits. For example, it is common to set New Years Resolutions focused on changing parts of your personality such as becoming more generous, kind, patient, or outgoing. In general, many experts agree that making real and lasting changes to broad traits can be exceedingly difficult. So if you are dissatisfied with certain aspects of your personality, is there really anything you can do to change? Some experts, including psychologist Carol Dweck, believe that changing the behavior patterns, habits, and beliefs the lie under the surface of the broad traits (e.g., introversion, agreeableness) is the real key to personality change. The Factors That Shape Personality To understand whether personality can be changed, we must first understand what exactly causes personality. The age-old nature versus nurture debate once again comes into play. Is personality shaped by our genetics (nature) or by our upbringing, experiences, and environment (nurture)?  In the past, theorists and philosophers often took a one-versus-the-other approach and advocated either for the importance of nature or nurture, but today most thinkers would agree that it is a mixture of the two forces that ultimately shape our personalities. Not only that, but the constant interaction between genetics and the environment can help shape how personality is expressed. For example, you might be genetically predisposed to being friendly and laid back, but working in a high-stress environment might lead you to be more short-tempered and uptight than you might be in a different setting. Dweck relates a story of identical twin boys separated after birth and reared apart. As adults, the two men married women with the same first names, shared similar hobbies, and had similar levels of certain traits measured on personality assessments. It is such examples that provide the basis for the idea that our personalities are largely out of our control. Instead of being shaped by our environment and unique experiences, these twin studies point to the power of genetic influences. Genetics is certainly important, but other studies also demonstrate that our upbringing and even our culture interact with our genetic blueprints to shape who we are. Focusing on the In-Betweens of Personality Might Be Key But Dweck suggests that personality change is still possible. Broad traits might be stable through life, but Dweck believes that it is our in-between qualities that lie under the surface of the broad traits that are the most important in making us who we are. It is those in-between qualities, she believes, that can be changed. So what exactly are these in-between parts of personality? Beliefs and belief systems, Dweck proposes, play a vital role in shaping personality below the level of the broad traits. While changing certain aspects of your personality might be challenging, you can realistically tackle changing some of the underlying beliefs that help shape and control how your personality is expressed.Other theorists have suggested that factors such as goals and coping strategies play a primary role in determining personality. For example, while you might have more of a Type A personality, you can learn new coping skills and stress management techniques that help you become a more relaxed person.   Peoples beliefs include their mental representations of the nature and workings of the self, of their relationships, and of their world. From infancy, humans develop these beliefs and representations, and many prominent personality theorists of different persuasions acknowledge that they are a fundamental part of personality, Dweck explained in a 2008 paper. Why focus on beliefs? While changing beliefs might not necessarily be easy, it offers a good starting point. Our beliefs shape so much of our lives, from how we view ourselves and others, how we function in daily life, how we deal with lifes challenges, and how we forge connections with other people. If we can create real change in our beliefs, it is something that might have a resounding effect on our behaviors and possibly on certain aspects of our personality. Take, for example, beliefs about the self including whether personal attributes and characteristics are fixed or malleable. If you believe your intelligence is a fixed level, then you are not likely to take steps to deepen your thinking. If, however, you view such characteristics as changeable, you will likely make a greater effort to challenge yourself and broaden your mind. Obviously, beliefs about the self do play a critical role in how people function, but researchers have found that people can change their beliefs in order to take a more malleable approach to self-attributes. In one experiment, students had a greater appreciation of academics, higher grade-point-averages, and a greater overall enjoyment of school after discovering that the brain continues to form new connections in response to new knowledge. Dwecks own research has demonstrated that how kids are praised can have an impact on their self-beliefs. Those who are praised for their intelligence tend to hold fixed-theory beliefs about their own personal attributes. These kids view their intelligence as an unchangeable trait; you either have it or you dont. Children who are praised for their efforts, on the other hand, typically view their intelligence as malleable. These kids, Dweck has found, tend to persist in the face of difficulty and are more eager to learn. So What Can You Really Do to Change Your Personality? Changing from an introvert to an extrovert might be extremely difficult (or even impossible), but there are things that the experts believe you can do to make real and lasting changes to aspects of your personality. Focus on changing your habits. Psychologists have found that people who exhibit positive personality traits (such as kindness and honesty) have developed habitual responses that have stuck. Habit can be learned, so changing your habitual responses over time is one way to create personality change. Of course, forming a new habit or breaking an old one is never easy and it takes time and serious effort. With enough practice, these new patterns of behavior with eventually become second nature.Change your self-beliefs. If you believe you cannot change, then you will not change. If you are trying to become more outgoing, but you believe that your introversion is a fixed, permanent, and unchangeable trait, then you will simply never try to become more sociable. But if you believe that your personal attributes are changeable, you are more likely to make an effort to become more gregarious.Focus on the process. Dwecks research has consistently shown that praising efforts rather than ability is essential. Instead of thinking Im so smart or Im so talented, replace such phrases with I worked really hard or I found a good way of solving that problem. By shifting to more of a growth mindset rather than a fixed mindset, you may find that it is easier to experience real change and growth.  Fake it till you make it. Positive psychologist Christopher Peterson realized early on that his introverted personality might have a detrimental impact on his career as an academic. To overcome this, he decided to start acting extroverted in situations that called for it, like when delivering a lecture to a class full of students or giving a presentation at a conference. Eventually, these behaviors simply become second-nature. While he suggested that he was still an introvert, he learned how to become extroverted when he needed to be. A Word From Verywell Personality change might not be easy, and changing some broad traits might never really be fully possible. But researchers do believe that there are things you can do to change certain parts of your personality, the aspects that exist beneath the level of those broad traits, that can result in real changes to the way you act, think, and function in your day-to-day life.

My Dream Job in Promoting Green Communities - Free Essay Example

At this point in time, I find the world of sustainability overwhelming. There are some many possibilities in so many fields of study and application. Every time I research sustainable practices I gain more knowledge that can be applied in so many facets. So, I not sure currently what my dream-job is the field of sustainability. I know that I want to be an advocate for a sustainable world and I like the idea of promoting green communities. I like the idea of â€Å"farm to table† services and sustainable forms of food production. But I also, like the idea of natural methods of remediating water and soil resources. Many of the interests in the sustainable field that I listed, I do not currently have the skill-set to apply. To complete the homework assignment, I did choose an interest that fits the skill-set that I currently possess and applies the theories of sustainability in an educational sense. Nature-based learning is not just about teaching future generations to be good stewards of the earth. It is about the development of the whole child through the interconnections of their earthly origins. The methodologies of nature-based learning are about the total emersion of academic development and human development. It is a venue in which all learning styles are addressed, and the 5-senses are actively engaged. The nature-based curriculum provides a cornucopia of learning pathways where in which a child becomes a full participant in their own educational process and are confidently able to own their own deductions. The outdoor classroom engages the mind, body and soul. Children are given the freedom to use the boundless discoveries in an outdoor environment to develop their imagination, ability to self-express, gross and fine motor skills, critical thinking, social interaction and other learning developmental mile-stones. Nature-based learning is not only an application for holistic learning for the learner it is also, a vehicle for preparation for living in a sustainable world. This current generation is on the cusp of a future in which sustainable practices will be a normal application in every aspect of life. Through nature-based learning, children will be able to make direct physical connections with the elements that make up natural and man-made resources that are used daily. Children will also develop a greater understanding of the interconnections and impacts that human populations have on the earth’s ecological processes. The oracle of this holistic approach to learning is the nature-based educator. The Nature-Based Educator is responsible for building curriculum that meets academic requirements for each developmental level; however, they must also go beyond the standard application and presentation. Nature based curriculum walks a delicate balance between allowing a child the freedom to discover their own understanding and careful, constructive guidance from the educator. The nature-based educator must be willing spend most of their teaching time outdoors. They must be adaptive in creatively intertwining standard teaching curriculum with the overriding theme of outdoor education and hands-on learning. Curriculum Development and preparation for nature-based is more intense then the standard classroom curriculum. This is based on the fact that the nature-based educator will be working in an environment with unpredictable variables. The classroom is the great-outdoors, with no walls for spatial boundaries. The nature-based educator will need to build a curriculum that utilizes what nature has to offer on any given day. Safety and protocols must also be built into each activity. Again, safety is another facet in which nature-based education walks a fine-line. The overriding philosophy of the outdoor classroom is to give children the freedom to explore. This means, climbing trees, rolling logs, playing in a stream, building forts and so on – all of which are considered potential hazards in a standard classroom setting. A nature-based educator must be willing to embrace the elements of the great out-doors and be willing to turn any unpredictable natural phenomena into a learning opportunity. It is with great enthusiasm that I submit my application for the position of Senior Nature-Based Curriculum Developer. I have extensive experience in science, nature and recreational education program/curriculum development, presentation and training. My past job experiences have given me a wide variety of program development and facilitation in the classroom, outdoor nature education, and recreational environments. I have worked with all age levels including infants to seniors. I also, have significant experience in customer relations and recreation/park operations.